First step towards synthetic carbon dioxide fixation in living cells

January 2, 2024 This article has been reviewed according to Science X's editorial process and policies . Editors have highlighted the following attributes while ensuring the content's credibility: fact checked peer reviewed publication trusted source proofread by Max Planck Society Synthetic biology offers the opportunity to build biochemical pathways for the capture and conversion of carbon dioxide (CO 2 ).

Researchers at the Max Planck Institute for Terrestrial Microbiology have developed a synthetic biochemical cycle that directly converts CO 2 into the central building block Acetyl CoA. The researchers were able to implement each of the three cycle modules in the bacterium E.coli, which represents a major step towards realizing synthetic CO 2 fixing pathways within the context of living cells.

Developing new ways to capture and convert CO 2 is key to tackling the climate emergency. Synthetic biology opens avenues for designing new to nature CO 2 fixation pathways that capture CO 2 more efficiently than those developed by nature. However, realizing those new to nature pathways in different in vitro and in vivo systems is still a fundamental challenge.

Researchers in Tobias Erb's group have now designed and constructed a new synthetic CO 2 fixation pathway, the so called THETA cycle. It contains several central metabolites as intermediates and has the central building block, acetyl CoA, as its output. This characteristic makes it possible to be divided into modules and integrated into the central metabolism of E.

coli. The entire THETA cycle involved 17 biocatalysts and was designed around the two fastest CO 2 fixing enzymes known to date: crotonyl CoA carboxylase/reductase and phosphoenolpyruvate carboxylase. The researchers found these powerful biocatalysts in bacteria. Although each of the carboxylases can capture CO 2 more than ten times faster than RubisCO, the CO 2 fixing enzyme in chloroplasts, evolution itself has not brought these capable enzymes together in natural photosynthesis.

The THETA cycle converts two CO 2 molecules into one acetyl CoA in one cycle. Acetyl CoA is a central metabolite in almost all cellular metabolism and serves as the building block for a wide array of vital biomolecules, including biofuels, biomaterials, and pharmaceuticals, making it a compound of great interest in biotechnological applications.

Upon constructing the cycle in test tubes , the researchers could confirm its functionality. Then, the training began: through rational and machine learning guided optimization over several rounds of experiments, the team was able to improve the acetyl CoA yield by a factor of 100. In order to test its in vivo feasibility, incorporation into the living cell should be carried out step by step.

To this end, the researchers divided the THETA cycle into three modules, each of which was successfully implemented into the bacterium E. coli. The functionality of these modules was verified through growth coupled selection and/or isotopic labeling. "What is special about this cycle is that it contains several intermediates that serve as central metabolites in the bacterium's metabolism.

This overlap offers the opportunity to develop a modular approach for its implementation," explains Shanshan Luo, lead author of the study. "We were able to demonstrate the functionality of the three individual modules in E. coli. However, we have not yet succeeded in closing the entire cycle so that E.

coli can grow completely with CO 2 ," she adds. Closing the THETA cycle is still a major challenge, as all of the 17 reactions need to be synchronized with the natural metabolism of E. coli, which naturally involves hundreds to thousands of reactions. However, demonstrating the whole cycle in vivo is not the only goal the researcher emphasizes.

"Our cycle has the potential to become a versatile platform for producing valuable compounds directly from CO 2 through extending its output molecule, acetyl CoA," says Shanshan Luo. "Bringing parts of the THETA cycle into living cells is an important proof of principle for synthetic biology ," adds Tobias Erb.

"Such modular implementation of this cycle in E. coli paves the way to the realization of highly complex, orthogonal new to nature CO 2 fixation pathways in cell factories. We are learning to completely reprogram the cellular metabolism to create a synthetic autotrophic operating system for the cell." The study is published in the journal Nature Catalysis .

More information: Shanshan Luo et al, Construction and modular implementation of the THETA cycle for synthetic CO 2 fixation, Nature Catalysis (2023). DOI: 10.1038/s41929 023 01079 z Journal information: Nature Catalysis Provided by Max Planck Society.